Results
| PMID | 20172870 |
| Gene Name | TBPL2 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 66 |
| Population details | 66 (35 patients with histologically confirmed endometriosis, 31 patients without endometriosis) |
| Sex | Female |
| Associated genes | TRX, TBP-2 |
| Other associated phenotypes |
Endometriosis |
|
Hum Reprod. 2010 May;25(5):1251-8. doi: 10.1093/humrep/deq027. Epub 2010 Feb 19. Seo, Seok Kyo| Yang, Hyo In| Lee, Kyung Eun| Kim, Hye Yeon| Cho, Sihyun| Choi, Young Sik| Lee, Byung Seok Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University, College of Medicine, 146-92 Dogok-dong, Gangnam-gu, Seoul 135-720, Korea. BACKGROUND: Oxidative stress is considered to be involved in the establishment and development of endometriosis. Thioredoxin (TRX) is an endogenous redox regulator that protects cells against oxidative stress, and TRX-binding protein-2 (TBP-2) is a negative regulator of TRX in the biological function and expression. The aim of this study was to investigate the roles of TRX and TBP-2 in the pathophysiology of endometriosis. METHODS: A total of 35 patients with histologically confirmed endometriosis and 31 patients without endometriosis participated in this study. Real-time polymerase chain reaction was used to quantify TRX and TBP-2 mRNA levels, and immunohistochemistry (IHC) was used to assess TRX and TBP-2 protein localization in the endometrium. Serum and peritoneal fluid levels of TRX and TBP-2 were measured using a specific commercial ELISA. RESULTS: There were no significant differences in TRX mRNA levels in the endometrium of patients with endometriosis and the control groups. However, TBP-2 mRNA levels in the endometrium were lower, and the TRX to TBP-2 ratio was higher in patients with endometriosis than in the control group. In particular, the TRX to TBP-2 ratio was significantly higher during late secretory and menstrual phase in patients with endometriosis compared with the control group. IHC studies also showed the decreased TBP-2 immunoreactivity in patients with endometriosis compared with the control group. There was no correlation between TRX and TBP-2 mRNA levels in patients with endometriosis, whereas TRX mRNA levels were positively correlated with TBP-2 mRNA levels in the control group. There were no significant differences between the two groups in TRX and TBP-2 levels in serum or peritoneal fluid. CONCLUSIONS: Aberrant expression of TRX and TBP-2 in the endometrium may be associated with the establishment of endometriosis. Mesh Terms: Adult| Ascitic Fluid/metabolism| Base Sequence| Carrier Proteins/blood/*genetics/*metabolism| Case-Control Studies| DNA Primers/genetics| Endometriosis/etiology/*genetics/*metabolism| Endometrium/metabolism| Female| Humans| Immunohistochemistry| |
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